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KMID : 0616120220530040207
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Korean Journal of Pharmacognosy
2022 Volume.53 No. 4 p.207 ~ p.212
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Antioxidant Activity of Ethyl acetate Fraction of Ixeris dentata (Thunb.) Nakai in Caenorhabditis elegans
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Kim Dae-Keun
Kim Jun-Hyeong
Kwon Kang-Mu
Hwang In-Hyun
Ju Nam-Jin
Kim Dae-Keun
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Abstract
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Through the Caenorhabditis elegans model system, the antioxidant activity of methanol extract of Ixeis dentata was investigated. The ethyl acetate soluble fraction of the I. dentata methanol extract showed the best DPPH radical scavenging activity. The ethyl acetate fraction was measured for the activity of superoxide dismutase (SOD), catalase, and oxidative stress tolerance by using C. elegans along with reactive oxygen species (ROS) level. In addition, to confirm that the regulation of the stress response gene is responsible for the increased stress tolerance of C. elegans treated by the ethyl acetate fraction, SOD-3 expression was measured using a transgenic strain (CF1553). As a result, the ethyl acetate fraction increased SOD and catalase activity, and decreased ROS accumulation in a dose-dependent manner. In addition, the ethyl acetate fraction-treated CF1553 worm showed higher SOD-3::GFP intensity than the control worm.
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KEYWORD
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Ixeis dentata, Caenorhabditis elegans, Antioxidant activity, Catalase, SOD
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